Journal: The FASEB Journal

Article Title: Circadian period 2: a missing beneficial factor in sickle cell disease by lowering pulmonary inflammation, iron overload, and mortality

doi: 10.1096/fj.201900246RR

Figure Lengend Snippet: Genetic deletion of Per1/Per2 enhances pulmonary leukocyte infiltration and lung tissue damage, which contribute to an early mortality in SCD. A) Schematic of SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. B) Images of irradiated male mice ∼16 wk post-BMT. C) Increased mortality was identified beginning 42 d and up to 112 d after irradiation and hematopoietic adoptive transfer in female SCD BM transplanted to Per1/Per2 dKO mice. D) Panel of histologic stains, hematoxylin and eosin (H&E), and Ly6-positive leukocytes as indicated with red arrows in SCD or WT BM transplanted to WT or Per1/Per2 dKO mice. E) Semiquantification of histologic assessment of lung isolated from SCD or WT phenotypic mice with or without Per1/Per2. F) Quantification of leukocytes in ×20 field in whole lung sections isolated from SCD and WT BM transplant mice; n = 5 mice/group. ND, not determined. *P < 0.05, SCD → WT compared with WT → WT mice, **P < 0.01, SCD → WT compared with SCD → Per1/Per2 dKO mice.

Article Snippet: Sections were incubated with Ly6 primary antibody (rat monoclonal anti-mouse, 1:500 dilution in blocking solution; BD Biosciences, San Jose, CA, USA) overnight at 4°C.

Techniques: Irradiation, Adoptive Transfer Assay, Isolation

Journal: Current Issues in Molecular Biology

Article Title: The Occluded Epitope Residing in Spike Receptor-Binding Motif Is Essential for Cross-Neutralization of SARS-CoV-2 Delta Variant

doi: 10.3390/cimb44070195

Figure Lengend Snippet: Western blot analysis of 40591-MM43 mAb to spike RBD-His recombinant protein (WT, Delta, and W436R). Proteins (strips 1–3 WT; strips 4–6 Delta; strip 7-9 W436R) on the blot were probed with SARS-CoV-2 spike RBD mAb (40591-MM43) (strips 1, 4, and 7); SARS-CoV-2 spike RBD mouse pAb (4059 2-MP01) (strips 2, 5, and 8); mouse anti-6x His mAb (strips no. 3, 6, and 9) followed by goat anti-mouse Igs conjugated with HRP.

Article Snippet: After washing, the membranes were incubated with an HRP-conjugated goat anti-mouse immunoglobulin antibody (dilution 1:5,000 in blocking solution) for 1 h. The membranes were washed, and bands were visualized using KPL LumiGLO ® Chemiluminescent Substrate System (Seracare, Milford, MA, USA) (Cat. 5430-0040).

Techniques: Western Blot, Recombinant, Stripping Membranes